Blocking Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Staining:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Electron Microscopy:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Cell Isolation:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Magnetic Cell Separation:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Single Cell:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Real-time Polymerase Chain Reaction:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
SYBR Green Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Enzyme-linked Immunosorbent Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Western Blot:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Membrane:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Article Title: FoxO1 promotes high glucose-induced inflammation and cataract formation via JAK1/STAT1.
Article Snippet: Purpose To investigate whether in diabetic cataract (DC), FoxO1 regulates high glucose (HG)-induced activation of NLRC4/ IL-6 inflammatory mediators in human lens epithelial cells (SRA01/04) via the JAK1/STAT1 pathway, leading to cataract formation.. Methods Expression levels of FoxO1, inflammatory factor IL-6 and inflammatory vesicle NLRC4 were examined in SRA01/04 under high glucose (HG) stress at 25–150 mM.. Rat lenses were also cultured using HG medium with or without the addition of the FoxO1 inhibitor AS1842856 and the JAK1 agonist RO8191.
Cell Culture:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Article Title: The ARHGAP10-202aa protein encoded by circARHGAP10 promotes skeletal muscle development and regeneration.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.
Software:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Incubation:Article Title: The ARHGAP10-202aa protein encoded by circARHGAP10 promotes skeletal muscle development and regeneration.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.
Article Title: FoxO1 promotes high glucose-induced inflammation and cataract formation via JAK1/STAT1.
Article Snippet: Purpose To investigate whether in diabetic cataract (DC), FoxO1 regulates high glucose (HG)-induced activation of NLRC4/ IL-6 inflammatory mediators in human lens epithelial cells (SRA01/04) via the JAK1/STAT1 pathway, leading to cataract formation.. Methods Expression levels of FoxO1, inflammatory factor IL-6 and inflammatory vesicle NLRC4 were examined in SRA01/04 under high glucose (HG) stress at 25–150 mM.. Rat lenses were also cultured using HG medium with or without the addition of the FoxO1 inhibitor AS1842856 and the JAK1 agonist RO8191.
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